Reporter

Part:BBa_K1135004:Design

Designed by: Ranvir Chaudhri   Group: iGEM13_UCL_PG   (2013-09-20)


PcstA promoter-RBS-mKeima-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 720
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mKeima had to have one restriction site removed to fit the biobrick standard. Other than that it was easy.


Source

Promoter, RBS and Terminator were each in the registry. My team added the mKeima.

References